Twodimensional cell culture techniques, in which cells are grown on. Powering ex vivo tissue models in microfluidic systems. Thereby 3d cell culture realistically models in vivo tissue conditions and processes, and provides in vivo like responses. The transition from 2d to 3d cell culture techniques is an important step in a trend towards better biomimetic tissue models. Stereolithographic hydrogel printing of 3d microfluidic cell culture chips zhang, rujing publication date. The combination of microfluidic technology with 3d cell culture offers great. Vasculature was the most modelled tissue, followed by brain and liver. Recent developments have opened the door to a new set of opportunities for microfluidic systems, in the field of tissue and organ engineering. Microfluidic culture models of tumor angiogenesis tissue. Microfluidic scaffolds for tissue engineering nature. Medical and industrial applications of microfluidicbased celltissue culture and organsonachip author. These stresses, especially if sustained, can induce changes in cell structure, contractile function, and gene expression. The 3dprinting enabled microassembly allows facile creation of a novel 3d culture system for electrotransfection, which can be employed for versatile gene delivery and cellular engineering, as well as building in vivo like tissue models for fundamentally studying cellular regulation mechanisms at.
The combination of microfluidic technology with 3d cell culture offers great potential for in vivolike tissuebased applications, such as the emerging organonachip system. Microfluidic systems have emerged as revolutionary new platform technologies for a range of applications, from consumer products such as inkjet printer cartridges to labonachip diagnostic systems. Amongst them, tissue engineering specializes in repairing damaged tissues by injecting new one generated by 3d cell culture. Recent advances of biologically inspired 3d microfluidic hydrogel. Recent developments in microfluidics have demonstrated the enormous potential of microscale cell culture for biology studies and recognized as instrumental in performing rapid and efficient experiments on smallsample volumes. The application of an optically switched dielectrophoretic odep force for the manipulation and assembly of cellencapsulating alginate microbeads in a microfluidic perfusion cell culture system for bottomup tissue engineering. Tumor cell viability, proliferation, and morphology. Microfluidic assay for simultaneous culture of multiple.
Microfluidic strategies for efficient mass transport or vascularization. After a brief description of the background of tissue engineering, different application areas of microfluidic devices are examined. Utilization of these microfluidic tumor models will help reveal the importance of the transport of chemical and cellular factors in tumor angiogenesis, and provide a test bed. Thus, a plethora of studies in bone tissue engineering are conducted using both collagen and fibrin composites. While it was once categorized as a subfield of biomaterials, having grown in scope and. We examine the transport phenomena associated with gradients of soluble factors and oxygen in a microfluidic device for coculture. Microfluidic cell culture technologies offer the ability to create more relevant in vitro environments for preclinical studies using liver hepatocytes. Conventional cell culture and tissue engineering methods. By integrating tissue engineering and microfluidic technologies, it will be possible to develop tumormimetic culture environments with embedded microvascular structures. Comparison of biocompatibility and adsorption properties.
Through a combination of cell biology, engineering, and biomaterial technology, the microenvironment of the chip simulates that of the organ in terms of tissue interfaces and mechanical stimulation. In vivo applications include lung, liver, kidney, cardiac and other organ systems. However, the artifactual environment and the oversimplified structure of this onecelltype culture system do not mimic the in vivo dynamic nature and heterogeneous architecture. Studies of calcium alginate as a tissue scaffold have shown that it is appropriate for longterm culture and formation of functional tissue 5,27, compatible with macroscale moulding to form cell. Therefore, one needs to characterize the tissue scaffolds through experiments and theoretical simulations to achieve high cell viability. Demonstration of neuronal culture inside the microfabricated device and. L, nl, pl within artificially fabricated microsystems, and cell culture. A practical guide to microfluidic perfusion culture of. This is a highly inter disciplinary field and has received significant contributions from researchers from various fields of engineering and life. The microfluidic cell culture assay also allows the study of 3d cellcell interactions and cellecm interactions 25, especially for the evaluation of new synthesized biodegradable polymers. Although the recent advances in stem cell engineering have gained a great deal of.
Tissuebased models have a strong potential to recapitulate complex physiological mechanisms that are missing from models built from one cell type at a time. Microfabrication and microfluidics for tissue engineering. Highlights microfluidic cell culture systems are emerging for in vitro and in vivo applications. Current cell culture techniques commonly fail to adequately replicate physical loading observed in the native heart.
Microfluidic cell culture models for tissue engineering sciencedirect. Stereolithographic hydrogel printing of 3d microfluidic. Among these are methods for patterning cells, topographical control over cells and tissues, and bioreactors. With the development of tissue engineering, hydrogels have attracted extensive interest because of their advantageous properties similar to those of the native extracellular matrix ecm, such as high content of water, unique biocompatibility, biodegradability, as well as tunable. It constitutes the subject matter of significant biomedical engineering research. By recapitulating key features of the natural architecture and the multicellular arrangement of the native tissue, these platforms allow one to model and analyze at the molecular, cellular, and tissue level a wide range of interactions that. Tissue engineering has made great advances for achieving its goal to restore and improve tissueorgan function 1 3. Microfluidic cell culture systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organonchip systems. In vitro studies of vascular physiology have traditionally relied on cultures of endothelial cells, smooth muscle cells, and pericytes grown on centimeterscale plates, filters, and flow chambers. Pdmsbased microfluidic devices for cell culture mdpi. Microphysiologic cell culture systems are microfluidic platforms on which multiple in vitro tissues can communicate with each other via soluble metabolites that recirculate through a medium stream.
Engineered 3d tissue models for cellladen microfluidic. Sia, microfluidics for engineering 3d tissues and cellular microenvironments, microfluidic cell culture systems, 10. Engineering hydrogel tissue scaffolds can enhance this perfusion capacity that is necessary to maintain high cell viability 1821. Microfluidic hydrogels for tissue engineering iopscience. Tissue engineering is the use of a combination of cells, engineering, and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological tissues. Microfluidic cell culture integrates knowledge from biology, biochemistry, engineering, and physics to develop devices and techniques for culturing, maintaining, analyzing, and experimenting with cells at the microscale.
Patientspecific 3d microfluidic tissue model for multiple. The liver is the critical organ in detoxifying the body of xenobiotics such as biopharmaceuticals and environmental chemicals. Microfluidic cell culture models for tissue engineering. Schematics of 3d microfluidic ossified tissue culture system and procedures. A future goal of microfluidicsbased cell culture systems is organ replacement. Evaluating biomaterial and microfluidicbased 3d tumor models. However, it is important to note that almost all specialized cell types derived by differentiation of ipscs still exhibit immature phenotypes. This quality makes tissue models, and their developing microfluidic components, an important asset for research and development in the biomedical research enterprise. This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems. The main advantage of hydrogels for microfluidic cell cultures, however, is their. Cell culture on petri dish remains the gold standard in basic research for screening drug candidates during one of the most expensive and lengthy industrial product development. More recently, microfluidic stem cell culture systems have become popular avenues for modelling organs andor native tissue in. With the development of tissue engineering, hydrogels have attracted extensive interest because of their advantageous properties similar to those of the native extracellular matrix ecm,suchashighcontentofwater. This culture methods tries to reduce the gap between in vitro and in vivo drug testing models as much as possible.
Medical and industrial applications of microfluidicbased. Microfluidic technology for the generation of cell. Pdf an introductory overview of the use of microfluidic devices for tissue engineering is. Lin yh, yang yw, chen yd, wang ss, chang yh, wu mh. In order to grow large tissue models and organoids. A future goal of microfluidicsbased cell culture systems. Microfluidic cell culture systems are emerging for in vitro and in vivo applications. Cell culture models offer an easily accessible, highly reproducible, and.
Pdf microfabrication and microfluidics for tissue engineering. In recent years, microfluidic devices have emerged as a platform in which to culture tissue for various applications such as drug discovery, toxicity testing, and fundamental investigations of cellcell interactions. The 3dprinting enabled microassembly allows facile creation of a novel 3d culture system for electrotransfection, which can be employed for versatile gene delivery and cellular engineering, as well as building in vivo like tissue models for fundamentally studying cellular regulation mechanisms at the molecular level. Tissue engineering involves the use of a tissue scaffold for the formation of new viable tissue for a medical purpose.
A practical guide to microfluidic perfusion culture of adherent mammalian. Although such 2d in vitro models are less expensive than animal models and. Breast and lung tumor models comprise half of the developed models. Application of microfluidics in stem cell and tissue. Transport characteristics of microfluidic scaffold. A 3d model mimics the relevant invivo physiological conditions, allowing a cell culture to serve as an effective tool for drug discovery, tissue engineering, and the investigation of disease pathology. Microscale technologies for advanced stem cell culture. In vitro microfluidic models for neurodegenerative disorders. Traditional animal models or conventional cell cultures do not accurately. Microfluidics is a powerful tool for biological analysis. Cancer is a major cause of morbidity and mortality worldwide, with a disease burden estimated to increase over the coming decades. Sw620 and mkn74 cells were incubated in a hafibrin composite with 0. Microfluidic systems enable dynamic control over the cell microenvironment.
Since its early days in the 1970s, 3d cell culture has revealed important insights into mechanisms of tissue homeostasis and cancer, and accelerated translational research in cancer biology and tissue engineering. The organonachip ooac is in the list of top 10 emerging technologies and refers to a physiological organ biomimetic system built on a microfluidic chip. Tissue engineering collectively refers to efforts used to augment, repair or replace tissue or organ using cellular substitutes that have appropriate structural organization and function behavior. An introductory overview of the use of microfluidic devices for tissue engineering is presented. Permeability to diffusion of both small for example, biochemical factors and large for example, secreted extracellular matrix components molecules is essential for any matrix for 3d cell culture, and for the function of a microfluidic scaffold in particular fig. This paper is part of a special issue cell and tissue engineering in microsystems with guest editors sangeeta bhatia mit and. An organonachip is a 3d microfluidic cell culture system that simulates the activities, mechanics and physiological responses of entire organs orand organ systems. Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ nmr spectroscopy. Tissue engineering and regenerative medicine, a section of the journal. Microfluidic devices or microbioreactor has applied for tissue engineered and this technique for cell culturing to study the biological behavior of cells from single cells to multicells levels. Transport model for microfluidic device for cell culture.
Advances in the design of physiologically relevant structures and networks, fabrication processes for biomaterials suitable for in vivo use. Microfluidic technology for the generation of cell spheroids. Microfluidic threedimensional cell culture of stem cells for high. Microfluidic cell culture models for stem cell therapies. Other important on chip hydrogelbased tissue models constitute the heart. Physiological heart development and cardiac function rely on the response of cardiac cells to mechanical stress during hemodynamic loading and unloading. The introduction of microfluidic tools has revolutionized the study of vascular physiology by allowing researchers to create physiologically relevant culture models, at the same time greatly reducing. It merges microfluidics, a set of technologies used for the manipulation of small fluid volumes. Tissue models 47 a current opinion in biotechnology overview of the modelled organs of 87 articles which combine 3d cell culture with microfluidics since 2012.
Pdf neural tissue engineering aims at developing novel approaches for the treatment of diseases. Hydrogels in microfluidic systems for threedimensional tissue engineering. Microfluidic devices for studying heterotypic cellcell interactions and tissue specimen cultures under controlled microenvironments biomicrofluidics 5, 0406 2011. Pdf microfluidic systems for stem cellbased neural tissue. The application of microfluidic techniques on tissue. Disease models, drug discovery and safety testing represent in vitro applications. In particular, in the field of threedimensional culture models, a diffuse approach. Microfluidicbased cell culture is an area of research that keeps growing and gaining importance as a prominent technology, able to link scientific disciplines with. Tissue engineering has made great advances for achieving its goal to restore and improve tissueorgan function. Microfluidic devices in tissue engineering springerlink. Initially, microfluidic stem cell cultures have been used to investigate differentiation rates, cellcell interaction, cell behavior in coculture, as well as cellmatrix interactions. Disease heterogeneity and limited information on cancer biology and disease mechanisms are aspects that 2d cell cultures fail to address. My research combines tissue engineering and microfabrication to construct.
323 1087 269 1105 1261 1269 355 254 784 1503 1410 493 1636 658 431 463 988 972 101 1152 1220 162 620 519 943 363 433 936 859 328 1184